Description | This antibody displays high affinity and selectivity to RhoA and RhoB. Binding affinities of A5 to RhoAL63 and RhoBL63 were measured by competitive ELISA. The affinity of A5 to RhoAL63 is about 24.5 nM and the affinity of A5 to RhoBL63 is about 24 nM. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human |
Immunogen | Recombinant GST-fused Rho protein |
Affinity | 24.5 nM for RhoAL63 24 nM for RhoBL63 |
Isotype | IgG |
Expression Species | HEK293F or CHO cell line |
Conjugation | Unconjugated |
Purity | >95% |
Endotoxin | <1 EU/mg |
Form | Liquid |
Purification | Protein A purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Application | ELISA; WB |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |