| Description | E3 demonstrated specific interaction with the GTPγS-loaded Rho proteins. scFvE3 serves as a selective sensor for detecting RhoB activation in HeLa cells. It exhibits a preference for binding to the active conformation of RhoB. Notably, scFvE3 showed a higher affinity for RhoB (3.63 nM) compared to RhoA (22.9 nM). |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Recombinant GST-fused Rho protein |
| Affinity | 3.63 nM to RhoB 22.9 nM to RhoA |
| Isotype | IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; WB |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
