| Description | This product is a neutralizing antibody that binds to Severe Acute Respiratory Syndrome Coronavirus 2. S2X303 is capable of specifically binding with SARS-CoV-2 and shows a good capacity to neutralize the SARS-CoV-2. S2X303 exhibits unprecedented cross-reactivity compared to all other mAbs and can bind to a large number of variants. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | RBD receptor binding domain |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA |
| Application Notes | For ELISA experiments with NTD-targeted mAbs, 384-well Maxisorp plates were coated overnight at 4°C with 2 μg/mL of S glycoprotein in 20mM HEPES pH 8 and 150mM NaCl. Plates were slapped dry and blocked with Blocker Casein in TBS for one hour at 37°C. Plates were slapped dry and mAbs were serially diluted 1:5 in TBST with an initial concentration of 50 µg/ml. Plates were left for one hour at 37°C and washed 4x with TBST, then 1:5000 Goat anti-Human was added. Plates were left for one hour at 37°C and washed 4x with TBST, and then TMB Microwell Peroxidase was added. The reaction was quenched after 4 minutes with 1 N HCl and the A450 of each well was read using a BioTek plate reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
