| Description | This recombinant anti-SARS-CoV S antibody (clone 4S1) is against the SARS-CoV S protein. The epitope of 4S1 is located between amino acid residues 456-650 (fragment Se-e) of the spike protein. |
| Clonality | Monoclonal |
| Host Species | Chicken |
| Target Species | SARS-CoV |
| Immunogen | Recombinant S proteins |
| Epitope | Amino acid residues 456-650 of S protein |
| Function | Spike glycoprotein: May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity. Spike protein S1: Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes. Spike protein S2: Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis. |
| Isotype | Chicken IgY |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; IF; ICC |
| Application Notes | The binding ability of this antibody to S protein was detected by ELISA. The epitope on the S protein recognized by 4S1 was characterized by ELISA, Western blot and Immunofluorescent. 4S1 was tested with immunocytochemical staining in SARS-CoV-infected Vero E6 cell. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Post Translational Modification | The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested by cathepsin CTSL within endosomes. Specific enzymatic cleavages in vivo yield mature proteins. The precursor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins. Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor. The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes. |
