| Description | This recombinant human antibody (clone T7) recognizes TNF-α. This TNF-α specific recombinant antibody was generated by phage display from a human combinatorial antibody library. This antibody has been tested in use for ELISA and WB. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | TNF-α recombinant protein |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | WB; ELISA |
| Application Notes | The results of ELISA showed this antibody had no cross reactivity with other proteins, such as Glutathione-S-transferase (GST), Immunodominant Membrane Protein (IMP), and Fig Mosaic Virus Nucleic Capsid Protein (FMV NCP). The binding activities of this antibody with TNF-α could detected by ELISA and Western Blot. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
