| Description | This recombinant chicken antibody (clone A8) is more specific for Usutu virus E protein domain III than West nile virus (WNV) E protein. The binding specificity of this antibody to USUV E DIII was examined by ELISA and Western Blot. |
| Clonality | Monoclonal |
| Host Species | Chicken |
| Target Species | Usutu virus (USUV) |
| Immunogen | Purified Usutu virus envelope protein domain III |
| Epitope | E protein DIII from USUV group A, B, and I |
| Affinity | KD = 90 nM (USUV E protein DIII group A); KD = 100 nM (USUV E protein DIII group B); KD = 60 nM (USUV E protein DIII group I); KD = 3300 nM (WNV E protein DIII); |
| Isotype | Chicken IgY |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; SPR |
| Application Notes | The binding specificity of this antibody to Usutu virus envelope protein domain III was examined by ELISA and Western Blot. The affinity of A8 to domain III was examined using SPR. A8 saturated the virus Vienna-2001 strain at a concentration below 5 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | USUV E DIII |
| Alternative Name | Usutu virus E protein domain III; E protein; Usutu virus E; USUV E |
| Research Area | Microbiology |
| Related Disease | Encephalitis |
