| Description | This recombinant chicken antibody (clone A9) recognized both Usutu virus (USUV) and West nile virus(WNV) E protein domain III. The binding specificity of this antibody to USUV E DIII was examined by ELISA and Western Blot. |
| Clonality | Monoclonal |
| Host Species | Chicken |
| Target Species | Usutu virus (USUV); West nile virus (WNV) |
| Immunogen | Purified Usutu virus envelope protein domain III |
| Epitope | DIII of WNV and USUV (group A, B, I) E protein |
| Affinity | KD = 350 nM (USUV E protein DIII group A); KD = 380 nM (USUV E protein DIII group B); KD = 290 nM (USUV E protein DIII group I); KD = 100 nM (WNV E protein DIII); |
| Isotype | Chicken IgY |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; SPR |
| Application Notes | A9 saturated the virus Vienna-2001 strain at a concentration below 5 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | USUV E DIII |
| Alternative Name | Usutu virus E protein domain III; E protein; Usutu virus E; USUV E |
| Research Area | Microbiology |
| Related Disease | Encephalitis |
