RSPO1 Specific Neutra™ Antibody Products

RSPO1 acts as a ligand for leucine-rich repeat-containing G-protein coupled receptors, promoting the activation of the Wnt signaling cascade. RSPO1 serves as a crucial secreted stimulatory protein that triggers swift crypt cell proliferation and enhances the healing of intestinal epithelium in murine models, offering protection against chemotherapy-induced adverse reactions.

Its Gene ID: 284654, UniProtKB ID: Q2MKA7, and OMIM ID: 609595.

RSPO1 Pathway

The principal molecular role attributed to RSPO1 lies in their ability to activate the Wnt/β-catenin signaling pathway. By engaging with their cognate receptors LGR4/5/6, predominantly expressed by stem and progenitor cells, RSPO1 notably enhances Wnt/β-catenin signaling, particularly within these proliferative cellular domains. Given the widespread expression of LGR4/5/6 receptors and RSPO1 ligands in various organ-specific stem cell niches, RSPO1 exerts significant influence over stem cell regulation throughout the organism. Nevertheless, heightened RSPO1 expression levels have been documented across diverse cancer types affecting various organs with distinct stem cell hierarchies. Notably, the emerging oncogenic role of RSPO1 and its potential as a therapeutic target have garnered recognition and exploration in both preclinical and clinical contexts.

Fig. 1 The canonical Wnt pathway enhanced by RSPO stimulation.^{1, 4}

Applications of Anti-RSPO1 Antibodies

• RSPO1 Antibody Intervention Holds Promise as a Potential Strategy for Bone Disorders

RSPO1 protein plays pivotal roles in osteoblastic differentiation and bone formation. RSPO1 emerges as a mechanosensitive factor influencing bone formation. Continuous cyclic mechanical stretch (CMS) induces an upregulation of RSPO1 expression in mouse bone marrow mesenchymal stem cells (BMSCs), while in vivo, RSPO1 expression in BMSCs decreases in bones subjected to mechanical unloading. Conversely, administration of RSPO1 neutralizing antibody counteracts the heightened osteogenic differentiation and Wnt/β-catenin activity induced by CMS in BMSCs, effectively mitigating bone loss in mechanically unloaded mice. Thus, targeting RSPO1/Lgr4 holds promise as a novel approach for preventing and treating disuse osteoporosis in the future.

Fig. 2 Inhibition of enhanced osteogenic differentiation and Wnt/β-catenin activity by RSPO1 neutralizing antibody in BMSCs stimulated by CMS.^{2, 4}

• Implications of RSPO1 in Gonadal Development

In mammalian XX sex-reversal occurrences, Polled Intersex Syndrome (PIS) in goats and RSPO1 in humans are recognized genetic loci. RSPO1 demonstrates sexually dimorphic expression in goat gonads, predominantly in ovaries, unaffected by PIS mutation. Immunofluorescence analysis utilizing an anti-RSPO1 antibody reveals RSPO1 protein localization to germ cell membranes, notably during early differentiation and meiosis. RSPO2 and RSPO4 also exhibit sexually dimorphic expression patterns, with RSPO2 notably reduced in XX PIS-/- gonads. These observations suggest RSPO1's contribution to germ cell development and meiosis.

Fig. 3 Assessing RSPO1 antibody specificity through immunofluorescence analysis.³

Creative Biolabs provides a range of high-quality research-grade antibodies targeting RSPO1, specifically designed for studying therapeutic approaches for diverse conditions. Additionally, our team can be reached for access to personalized functional antibodies based on RSPO1.

REFERENCES

1. Ter Steege, Eline J., and Elvira RM Bakker. "The role of R-spondin proteins in cancer biology." Oncogene 40.47 (2021): 6469-6478.
2. Shi, Gui-Xun, et al. "Evidence of the role of R-Spondin 1 and its receptor Lgr4 in the transmission of mechanical stimuli to biological signals for bone formation." International journal of molecular sciences 18.3 (2017): 564.
3. Kocer, Ayhan, et al. "R-spondin1 and FOXL2 act into two distinct cellular types during goat ovarian differentiation." BMC Developmental Biology 8 (2008): 1-16. Distributed under Open Access license CC BY 2.0, without modification.
4. Distributed under Open Access license CC BY 4.0, without modification.