| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Angiopoietin Like 4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Mouse |
| Immunogen | Recombinant N-mANGPTL4 |
| Isotype | IgG2b |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; Neut |
| Application Notes | The antibody is recommended for detection of ANGPTL4 by ELISA, Neut assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ANGPTL4 |
| Alternative Name | Angiopoietin Like 4; Hepatic Fibrinogen/Angiopoietin-Related Protein; Peroxisome Proliferator-Activated Receptor (PPAR) Gamma Induced Angiopoietin-Related Protein; Hepatic Angiopoietin-Related Protein; PPARG Angiopoietin Related Protein; Fasting-Induced Adipose Factor; Angiopoietin-Related Protein 4; HFARP; Arp4; PGAR; |
| Gene ID | 51129 |
| UniProt | Q9BY76 |
| Research Area | Cardiovascular |
Figure 1 Anti-ANGPTL4 Monoclonal Antibody (V3S-0522-YC487) in ELISA
ELISA analysis of V3S-0522-YC487 was performed by coating with Recombinant Mouse Angiopoietin-like 4 Protein, CF (Cat#4880-AN-050).
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Figure 1 Anti-ANGPTL4 Monoclonal Antibody (V3S-0522-YC487) in ELISA
Figure 2 Anti-ANGPTL4 Monoclonal Antibody (V3S-0522-YC487) in WB
Western blot analysis of V3S-0522-YC487 was performed by with Recombinant Mouse Angiopoietin-like 4 Protein, CF (Cat#4880-AN-050).
V3S-0522-YC487 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
Figure 2 Anti-ANGPTL4 Monoclonal Antibody (V3S-0522-YC487) in WB
