| Description | This antibody demonstrates neutralizing properties against toxin A by effectively recognizing all toxin A repeat domains with a high affinity (KD<30 nM). Its mechanism of action involves neutralizing the toxin by inhibiting its binding to cell surfaces. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | C. difficile |
| Immunogen | A fragment of toxin A encompassing the full CWB domain. |
| Epitope | Amino acids 1800-2700 |
| Neutralization Mechanism | The most neutralizing monoclonal antibody did not abrogate CWB-domain association with CHO cell surfaces, but retarded the internalization or reorganization of the toxin at the cell surface. |
| Affinity | KD = 0.3 nM |
| Isotype | Mouse IgG2a, kappa |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| Application Notes | Neut: 0.2 μg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | C. difficile ToxA |
| Alternative Name | C. difficile ToxA |
| Research Area | Cell Biology |
| Related Disease | Bacterial disease |
