| Description | The mouse recombinant monoclonal antibody was expressed by a hybridoma cell line. It exhibits cross-reactivity with human and chimpanzee CD19 only, but not with cynomolgus, dog, rabbit, hamster, mouse, and other species investigated. The antibody blocks B-cell activation without causing B-cell depletion. The antibody shows a significant inhibition of proliferation of primary B- cells from three donors stimulated with anti-IgM and IL-4. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Chimpanzee |
| Immunogen | CD19-transfected HEK293 cells. |
| IC50 | 0.15 ug/mL |
| EC50 | 1.45 nM |
| Isotype | Mouse IgG1, kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | FC; Inhib |
| Application Notes | FC: 2.5-10 μg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD19 |
| Alternative Name | CD19 Molecule; B-Lymphocyte Surface Antigen B4; T-Cell Surface Antigen Leu-12; Differentiation Antigen CD19; CD19 Antigen; B-Lymphocyte Antigen CD19; CVID3; B4 |
| Gene ID | 930 |
| UniProt | P15391 |
| Related pathway | PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling and Complement cascade |
| Research Area | Signal Pathway |
| Related Disease | Autoimmune disease |
