| Description | This product is a human monoclonal antibody that bind to the T-cell co-inhibitor ligand programmed death-ligand1 (PD-L1) protein. The antibody is useful for blocking or neutralizing PD-L1 activity. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Cynomolgus |
| Immunogen | A fragment of PD-L1 (aa 19-239). |
| Epitope | The epitope is located in the amino acids region 19-239. |
| Affinity | 20.8 pM-417 pM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FC; Neut; FuncS |
| Application Notes | Blocking of PD-L1 in a T-Cell/APC Luciferase Reporter Assay This is a bioassay developed to measure T cell signaling induced by interaction between APC and T cells by utilizing a mixed culture derived from two mammalian cell lines: Jurkat cells and Raji cells. The cells were transduced with the Cignal Lenti AP-1 Luc Reporter. Raji cells were transduced with human PD-L1 gene (amino acids 1-290). In this bioassay, antibodies blocking the PD1/PD-L1 interaction rescue T-cell activity by disabling the inhibitory signaling and subsequently leading to increased AP1-Luc activation. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
