Recombinant Anti-CD3 Antibody (V3S-0522-YC3153) (CAT#: V3S-0522-YC3153)

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Datasheet

MSDS

COA

Sub Cat Isotype Application  
V3S-1124-YC1275 IgG FC; IHC Inquiry
V3S-0522-YC3155 IgG FC Inquiry
V3S-0622-YC1283 IgG ELISA Inquiry
V3S-0622-YC2976 IgG2b ELISA Inquiry
V3S-0622-YC2978 IgG ELISA Inquiry
V3S-0622-YC4317 IgG1 ELISA; FC Inquiry
V3S-0622-YC75 IgG1 ELISA; FC Inquiry
V3S-0822-YC2677 IgG2a kappa Stim; Activ; Dep; Inhib; FC Inquiry
V3S-0822-YC2678 IgG1 kappa Inhib; FC; FuncS Inquiry
V3S-1022-YC184 IgG2a ELISA Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Cluster of differentiation 3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application FC; IHC
Application Notes The antibody is recommended for detection of CD3 by FC, IHC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CD3
Alternative Name T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
Gene ID 919
UniProt P20963
Research Area Immunology; Stem Cell
For research use only, not directly for clinical use.
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