| Description | This product is a recombinant chimeric (human/mouse) monoclonal antibody provided by Creative Biolabs. The monoclonal antibody is capable of specifically binding to lectin-like transcript 1 (LLT1). |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; DB; WB |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human CLEC2D Monoclonal Antibody (V3S-0622-YC5705-Fc silenced) in DB
Dot Blot analysis of V3S-0622-YC5705 (Fc silenced) was performed by coating with human CLEC2D protein (CLD-H5253, acrobiosystems).
V3S-0622-YC5705-Fc silenced incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
Figure 1 Anti-Human CLEC2D Monoclonal Antibody (V3S-0622-YC5705-Fc silenced) in DB
Figure 2 Anti-Human CLEC2D Monoclonal Antibody (V3S-0622-YC5705-Fc silenced) in WB
Western blot analysis of V3S-0622-YC5705 (Fc silenced) was performed by loading human CLEC2D protein (CLD-H5253, acrobiosystems) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC5705-Fc silenced and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
Figure 2 Anti-Human CLEC2D Monoclonal Antibody (V3S-0622-YC5705-Fc silenced) in WB
Figure 3 Anti-Human CLEC2D Monoclonal Antibody (V3S-0622-YC5705-Fc silenced) in ELISA
ELISA analysis of V3S-0622-YC5705 (Fc silenced) was performed by coating with human CLEC2D protein (CLD-H5253, acrobiosystems). Then blocked with BSA and incubated with V3S-0622-YC5705-Fc silenced. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-Human CLEC2D Monoclonal Antibody (V3S-0622-YC5705-Fc silenced) in ELISA
Figure 4 Anti-Human CLEC2D Monoclonal Antibody (V3S-0622-YC5705-Fc silenced) in FC
Flow cytometry analysis of Daudi cells with purified antibody V3S-0622-YC5705-Fc silenced (TE221587-2, Blue). Cells were incubated with V3S-0622-YC5705-Fc silenced (5 μg/5x10^5 cells) for 30 min at 4°C. The secondary antibody used was FITC-Goat Anti-Human IgG Fc antibody (ab97224, abcam) for 30 min at 4°C.
Figure 4 Anti-Human CLEC2D Monoclonal Antibody (V3S-0622-YC5705-Fc silenced) in FC
