Recombinant Anti-CLEC2D Antibody (V3S-0622-YC1409) (CAT#: V3S-0622-YC1409)

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  • fig1
    Figure 1 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in WB
  • fig1
    Figure 2 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in ELISA

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from mouse, which can specifically recognize CLEC2D. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Isotype IgG1

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; FC; ELISA
Application Notes The antibody is recommended for detection of CLEC2D by FC, ELISA assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CLEC2D
Alternative Name CLEC2D; C-type lectin domain family 2, member D; CLAX; LLT1; OCIL; C-type lectin domain family 2 member D; LLT-1; C-type lectin related f; lectin-like transcript 1; lectin-like NK cell receptor; osteoclast inhibitory lectin; C-type lectin superfamily 2, member D
Gene ID 29121
UniProt Q9UHP7
Research Area Cell Biology

Tested Data

WB

Figure 1 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in WB

Western blot analysis of V3S-0622-YC1409 was performed by loading recombinant human CLEC2D protein.

V3S-0622-YC1409 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG

Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)

Figure 1 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in WB

ELISA

Figure 2 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in ELISA

ELISA analysis of V3S-0622-YC1409 was performed by coating with recombinant human CLEC2D protein. Then blocked with BSA and incubated with Mouse Anti-CLEC2D Recombinant Antibody (V3S-0622-YC1409). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in ELISA

For research use only, not directly for clinical use.
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