| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize CLEC2D. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; FC; ELISA |
| Application Notes | The antibody is recommended for detection of CLEC2D by FC, ELISA assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CLEC2D |
| Alternative Name | CLEC2D; C-type lectin domain family 2, member D; CLAX; LLT1; OCIL; C-type lectin domain family 2 member D; LLT-1; C-type lectin related f; lectin-like transcript 1; lectin-like NK cell receptor; osteoclast inhibitory lectin; C-type lectin superfamily 2, member D |
| Gene ID | 29121 |
| UniProt | Q9UHP7 |
| Research Area | Cell Biology |
Figure 1 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in WB
Western blot analysis of V3S-0622-YC1409 was performed by loading recombinant human CLEC2D protein.
V3S-0622-YC1409 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
Figure 1 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in WB
Figure 2 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in ELISA
ELISA analysis of V3S-0622-YC1409 was performed by coating with recombinant human CLEC2D protein. Then blocked with BSA and incubated with Mouse Anti-CLEC2D Recombinant Antibody (V3S-0622-YC1409). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Recombinant Mouse Anti-CLEC2D Antibody (V3S-0622-YC1409) in ELISA
