| Description | This is a monoclonal antibody of the IgG1 isotype with kappa light chain specificity. It is designed to target human interleukin-8 (IL-8). Extensive epitope mapping studies have revealed its specific binding site to be located within the sequence KTYSKPFHPKFI of the IL-8 protein. The inhibitory activity of the antibody on calcium mobilization, quantified by IC₅₀, was measured at 0.1 ug/mL. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | A pool of recombinant human chemokines (IL-8, Gro-α, Gro-β, Gro-γ, and ENA-78). |
| Epitope | Amino acids 38 to 49, KTYSKPFHPKFI |
| Isotype | Mouse IgG1, kappa |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CXCL8 |
| Alternative Name | C-X-C Motif Chemokine Ligand 8; Monocyte-Derived Neutrophil Chemotactic Factor; Monocyte-Derived Neutrophil-Activating Peptide; Granulocyte Chemotactic Protein 1; Chemokine (C-X-C Motif) Ligand 8; Beta Endothelial Cell-Derived Neutrophil Activating Peptide; Lung Giant Cell Carcinoma-Derived Chemotactic Protein; Lymphocyte Derived Neutrophil Activating Peptide; Alveolar Macrophage Chemotactic Factor I; Tumor Necrosis Factor-Induced Gene 1; Neutrophil-Activating Peptide 1; T-Cell Chemotactic Factor; Interleukin 8; Emoctakin; GCP-1; MDNCF; MONAP |
| Gene ID | 3576 |
| UniProt | P10145 |
| Related pathway | MIF Mediated Glucocorticoid Regulation and TGF-Beta Pathway |
| Research Area | Signal Pathway |
| Related Disease | Inflammatory disease, Adult Respiratory Distress Syndrome and Melanoma |
