| Sub Cat | Immunogen | Epitope | Isotype | Application | |
|---|---|---|---|---|---|
| V3S-1124-YC797 | IgG kappa | Neut | |||
| V3S-0822-YC1088 | Human A431 membrane proteins | IgG1 | WB; IP; IHC; ICC; Neut | ||
| V3S-0822-YC2716 | Purified EGFR from A431 cells | IgG2a | Block; FuncS; IP; IHC-P; IF; FC; WB | ||
| V3S-0822-YC2717 | Purified EGFR from A431 cells | IgG1 | Block; WB; FuncS | ||
| V3S-1022-YC65 | In the aa 1-145 of EGFR. | IgG1 | Inhib | ||
| V3S-0522-YC5880 | In the C1 domain. | IgG | ELISA | ||
| V3S-0522-YC5881 | In the C2 domain. | IgG | ELISA | ||
| V3S-0522-YC5853 | IgG | FC | |||
| V3S-0522-YC5854 | HER2/HER3-transfected NIH 3T3 cell lines | IgG | FC | ||
| V3S-0522-YC5859 | A native EGFR antigen | IgG | WB; IF; IHC | ||
| V3S-0522-YC5886 | EGFR proteins | IgG | ELISA | ||
| V3S-0522-YC5901 | Cells expressing EGFR vIII mutant | IgG | FC | ||
| V3S-0522-YC6181 | IgG | ELISA; FC | |||
| V3S-0622-YC144 | IgG1 | ELISA; WB; IHC | |||
| V3S-0723-FY115 | IgG | ELISA; FC | |||
| V3S-0822-YC330 | A431 cultured cells | IgG2b kappa | FuncS | ||
| V3S-0522-YC8056 | Human epidermal growth factor receptor | IgG | WB | ||
| V3S-1022-YC556 | IgG | ELISA |
| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize EGFR. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Isotype | IgG kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | Neut |
| Application Notes | The antibody is recommended for detection of EGFR by Neut assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
