Description | This product is a monoclonal antibody that binds to the beta-hairpin of ERBB3. M-05-74 binds to the ERBB3 ECD in its active "open" conformation with improved kinetics, due to a better accessibility of the ERBB3 hairpin in its "open" conformation. M-05-74 binds to the ERBB3 ECD in its "closed" conformation with slower association rate constant. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Human |
Epitope | The epitope is located in the β-Hairpin, HER3 ECD epitope VYNKLTFQLEP and HER4 ECD epitope VYNPTTFQLE. |
Affinity | 2 nM for HER3 ECD and 150 nM for HER4 ECD |
Isotype | IgG1 kappa |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein G affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA; FC; IHC; Inhib; IP; WB; FuncS |
Application Notes | The antibody is recommended for detection of ERBB3 by ELISA, FC, IHC (1/20-1/200), Inhib, IP, WB (1/1000-1/5000), FuncS assays. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | ERBB3 |
Alternative Name | HER3; FERLK; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; Erb-b2 receptor tyrosine kinase 3; receptor tyrosine-protein kinase erbB-3; human epidermal growth factor receptor 3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 |
Gene ID | 2065 |
UniProt | P21860 |
Related pathway | PI3K/Akt pathway |
Research Area | Signal Pathway |