| Description | This product is a humanized antibody derived from mouse antibody M-05-74 against ERBB3. The antibody targets the ERBB3 dimerization domain (β-Hairpins of ERBB3). M-05-74 binds to the ERBB3 ECD in its active "open" conformation with improved kinetics, due to a better accessibility of the ERBB3 hairpin in its "open" conformation. M-05-74 binds to the ERBB3 ECD in its "closed" conformation with slower association rate constant. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Epitope | The epitope is located in the β-Hairpin, HER3 ECD epitope VYNKLTFQLEP. |
| Affinity | 23 nM |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FC; IHC; Inhib; IP; WB; FuncS |
| Application Notes | The antibody is recommended for detection of ERBB3 by ELISA, FC, IHC (1/20-1/200), Inhib, IP, WB (1/1000-1/5000), FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ERBB3 |
| Alternative Name | HER3; FERLK; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; Erb-b2 receptor tyrosine kinase 3; receptor tyrosine-protein kinase erbB-3; human epidermal growth factor receptor 3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3 |
| Gene ID | 2065 |
| UniProt | P21860 |
| Related pathway | PI3K/Akt pathway |
| Research Area | Signal Pathway |
