| Description | This is a neutralizing antibody clone 1F5 specific for F glycoprotein, derived from mouse. 1F5 recognizes the prefusion-specific, conserved quaternary epitope and locks F in its prefusion conformation. 1F5 has a specific affinity with NiV F and HeV F glycoprotein. 1F5 neutralizes NiV and HeV through inhibition of membrane fusion. This product has been detected by Enzyme-linked immunosorbent assay, Bio-layer interferometry and Inhibition assay. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Nipah virus (NiV), Hendra virus (HeV) |
| Immunogen | Prefusion stabilized NiV F ectodomain trimer |
| Epitope | A glycan-free quaternary epitope on the side of domain III of the HeV F globular head |
| Affinity | 1.47 nM (NiV F) 1.24 nM (HeV F) |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Inhib |
| Application Notes | Bio-layer interferometry (BLI): The equilibrium dissociation constants (Kd) of 1F5 to NiV F or HeV F is 1.47 and 1.24 nM, respectively. Inhib: The Ab broadly inhibited NiV-M, NiV-B and HeV with IC50 ranging between 0.2 and 1.3 µg/ml for 1F5-h1F5. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | F glycoprotein |
| Alternative Name | Fusion glycoprotein |
| Research Area | Infectious Disease |
| Related Disease | Respiratory illness, severe encephalitis |
