This is a neutralizing antibody clone 1F5 specific for F glycoprotein, derived from mouse. 1F5 recognizes the prefusion-specific, conserved quaternary epitope and locks F in its prefusion conformation. 1F5 has a specific affinity with NiV F and HeV F glycoprotein. 1F5 neutralizes NiV and HeV through inhibition of membrane fusion. This product has been detected by Enzyme-linked immunosorbent assay, Bio-layer interferometry and Inhibition assay.
Nipah virus (NiV), Hendra virus (HeV)
Prefusion stabilized NiV F ectodomain trimer
A glycan-free quaternary epitope on the side of domain III of the HeV F globular head
1.47 nM (NiV F) 1.24 nM (HeV F)
HEK293F or CHO cell line
Protein G purified
0.2 μM filtered
PBS, pH 7.4
Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.
ELISA; BLI; Inhib
Bio-layer interferometry (BLI): The equilibrium dissociation constants (Kd) of 1F5 to NiV F or HeV F is 1.47 and 1.24 nM, respectively. Inhib: The Ab broadly inhibited NiV-M, NiV-B and HeV with IC50 ranging between 0.2 and 1.3 µg/ml for 1F5-h1F5.