Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for HtrA serine peptidase 1. It can be used for HtrA serine peptidase 1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay, Flow Cytometry and Immunohistochemistry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Human, Mouse |
Immunogen | Purified His-tagged recombinant murine HtrA1 protease domain |
Isotype | IgG2a |
Expression Species | HEK293F or CHO Cell line |
Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Purified with Protein A or G affinity chromatography |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Application | WB; ELISA; FC; IHC |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | HTRA1 |
Alternative Name | HTRA1; HtrA serine peptidase 1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; serine protease HTRA1; IGFBP5-protease; protease, serine, 11 (IGF binding); high-temperature requirement A serine peptidase 1 |
Gene ID | 5654 |
UniProt | Q92743 |
Research Area | Cell Biology |
Related Disease | HtrA1-associated disorders, ocular disorders, and complement-associated disorders |