Anti-HTRA1 (protease domain) Neutralizing Antibody (V3S-0522-YC2276) (CAT#: V3S-0522-YC2276)

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  • fig1
    Figure 1 Anti-HTRA1 Monoclonal Antibody (V3S-0522-YC2276) in WB






Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize HtrA serine peptidase 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Immunogen The protease domain of mHtrAI
Isotype IgG1, κ


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application WB; ELISA; FC; Block
Application Notes The antibody is recommended for detection of HTRA1 by ELISA, FC, Block assays.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target HTRA1
Alternative Name HTRA1; HtrA serine peptidase 1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; serine protease HTRA1; IGFBP5-protease; protease, serine, 11 (IGF binding); high-temperature requirement A serine peptidase 1
Gene ID 5654
UniProt Q92743
Research Area Metabolism; Cell Biology
Related Disease HtrA1-associated disorder, Ocular disorder, Complement-associated disorder

Tested Data


Figure 1 Anti-HTRA1 Monoclonal Antibody (V3S-0522-YC2276) in WB

Western blot analysis of V3S-0522-YC2276 was performed by loading Recombinant Human HTRA1 protein (lane 1, 0.1 μg; lane 2, 0.3 μg; lane 3, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2276 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.

Figure 1 Anti-HTRA1 Monoclonal Antibody (V3S-0522-YC2276) in WB

For research use only, not directly for clinical use.
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