| Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for Influenza A virus HA. It can be used for IAV HA detection in Western Blot (WB), Enzyme-linked Immunosorbent Assay (ELISA), Immunohistochemistry (IHC), Neutralization (Neut). It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Influenza A Virus (IAV) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | DB; WB; ELISA; IHC; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in SDS-PAGE
SDS-PAGE analysis of V3S-1022-YC5958 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180-245 kDa.
Figure 1 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in SDS-PAGE
Figure 2 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in SEC-HPLC
SEC-HPLC analysis of V3S-1022-YC5958 shows that the purity is more than 95%.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in SEC-HPLC
Figure 3 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in ELISA
ELISA analysis of V3S-1022-YC5958 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with V3S-1022-YC5958. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in ELISA
Figure 4 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in ELISA
ELISA analysis of V3S-1022-YC5958 was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with V3S-1022-YC5958. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in ELISA
Figure 5 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC864) in WB
Western blot analysis of V3S-0522-YC3520 was performed by loading Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC3520 and HRP goat anti-mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 5 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC864) in WB
Figure 6 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC864) in WB
Western blot analysis of V3S-0522-YC3520 was performed by loading Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC3520 and HRP goat anti-mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 6 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC864) in WB
Figure 7 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in DB
Dot Blot analysis of V3S-1022-YC5958 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).
V3S-1022-YC5958 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 7 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in DB
Figure 8 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in DB
Dot Blot analysis of V3S-1022-YC5958 was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag).
V3S-1022-YC5958 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 8 Anti-IAV HA Monoclonal Antibody (V3S-1022-YC5958) in DB
