| Description | This is a neutralizing antibody clone AVFIuIgG03 specific for IAV HA (influenza A virus hemagglutinin), derived from human. AVFluIgG03 neutralizes the virus by directly blocking the attachment of the virus to the host cell. It has reasonably great potency and is able to neutralize 11 out the 17 pseudoviruses. This product has been determined using Enzyme-linked immunosorbent assay and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | IAV |
| Epitope | The epitope of AVFluIgG03 consisted of 17 external residues in the RBS at the membrane-distal end of the HA spike. A distinct set of critical residues consists of E130, S133, K156 and N159. |
| Neutralization Mechanism | AVFluIgG03 blocks the attachment of the virus to the host cell. |
| IC50 | 0.620±1.477 μg/mL |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| Application Notes | Neutralization assay (Neut): Twofold dilutions of each mAb was incubated with pseudovirus at the final volume of 100 μL at 37°C for 1 h. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IAV HA |
| Alternative Name | Hemagglutinin |
| Research Area | Infectious Disease |
| Related Disease | Influenza |
