| Description | This antibody is a neutralizing IgG clone 54-1G05 specific for IAV HA (Influenza A Virus hemagglutinin), derived from human. 54-1G05 from clonotype B is able to bind both group 1 (H1, H2, H5, H6, and H9) and group 2 (H3, H7, and H10) subtypes and exhibited high neutralization titers across both groups. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | IAV |
| Cross Reactivity | Group 1 (H1, H2, H5, H6, and H9) and group 2 (H3, H7, and H10) subtypes |
| Epitope | HA stem |
| Affinity | 961 nM |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | BLI; Neut |
| Application Notes | This antibody was tested by Bio-layer interferometry (BLI) and Neutralization assay. Bio-layer interferometry (BLI): The biosensors complexed with HA protein were allowed to associate with different concentrations of antibody (3200 nM, 800 nM, 200 nM and 50 nM). Neutralization assay (Neut): Pseudovirus was mixed with various dilutions of monoclonal antibodies for 45 minutes followed by addition to 293A cells in 96-well plates. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IAV HA |
| Alternative Name | Hemagglutinin |
| Research Area | Infectious Disease |
| Related Disease | Influenza |
