Recombinant Anti-IL17A (aa 63-87) Antibody (V3S-0522-YC6328) (CAT#: V3S-0522-YC6328)

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Description This product is a monoclonal antibody derived from Rat (Rattus norvegicus), which can specifically recognize Interleukin 17A. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Rat
Target Species Human, Cynomolgus, Mouse
Immunogen Recombinant human IL-17A (rhIL-17A) that had been expressed from adenoviral vectors in HEK 293 cell
Epitope Located in residues 63-87 of hIL-17A (PSVIWEAKCR HLGCINADG NVDYHM).
Affinity KD = 6.0 pM, determined by surface plasmon resonance.
Isotype IgG1


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application ELISA; ECL
Application Notes The antibody is recommended for detection of IL17A by ELISA, ECL assays.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target IL17A
Alternative Name IL17A; interleukin 17A; IL17; CTLA8; IL-17; IL-17A; interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
Gene ID 3605
UniProt Q16552
Research Area Cardiovascular; Immunology
Related Disease Inflammatory, Autoimmune, Proliferative disorder
For research use only, not directly for clinical use.
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