| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize INHBB. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Xenopus laevis |
| Immunogen | Recombinant mature Xenopus laevis activin B |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FuncS |
| Application Notes | The antibody is recommended for detection of INHBB by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human INHBB Monoclonal Antibody (V3S-0622-YC1951) in WB
Western blot analysis of V3S-0622-YC1951 was performed by loading Human Inhibin beta B Protein (His Tag).
V3S-0622-YC1951 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Figure 1 Anti-Human INHBB Monoclonal Antibody (V3S-0622-YC1951) in WB
Figure 2 Anti-Human INHBB Monoclonal Antibody (V3S-0622-YC1951) in DB
Dot Blot analysis of V3S-0622-YC1951 was performed by coating with Human Inhibin beta B Protein (His Tag).
V3S-0622-YC1951 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat-Anti-Mouse IgG (H+L)
Figure 2 Anti-Human INHBB Monoclonal Antibody (V3S-0622-YC1951) in DB
Figure 3 Anti-Human INHBB Monoclonal Antibody (V3S-0622-YC1951) in ELISA
ELISA analysis of V3S-0622-YC1951 was performed by coating with Human Inhibin beta B Protein (His Tag). Then blocked with BSA and incubated with V3S-0622-YC1951. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-Human INHBB Monoclonal Antibody (V3S-0622-YC1951) in ELISA
