| Description | This product is a mouse monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog. It can be used for KIT detection in Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC), Neutralization Assay (Neut). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | c-Kit/CD117 antibody was raised against a recombinant protein corresponding to the extracellular domain of human cKit. |
| Epitope | Within the ECD domain. |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purity | >95%, determined by SDS-PAGE |
| Purification | Protein A purified |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB; IP; FC; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | KIT |
| Alternative Name | KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein |
| Gene ID | 3815 |
| UniProt | P10721 |
| Research Area | Cancer Research |
