| Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for Microtubule Associated Protein Tau. It can be used for Microtubule Associated Protein Tau detection in Western Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry and Immunoprecipitation. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant human tau |
| Isotype | IgG2b |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | DB; WB; ELISA; IHC; IP |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-MAPT Monoclonal Antibody (V3S-1022-YC2673) in DB
Dot Blot analysis of V3S-1022-YC2673 was performed by coating with human MAPT protein (His tag).
V3S-1022-YC2673 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 1 Anti-MAPT Monoclonal Antibody (V3S-1022-YC2673) in DB
Figure 2 Anti-MAPT Monoclonal Antibody (V3S-1022-YC2673) in WB
Western blot analysis of V3S-1022-YC2673 was performed by loading human MAPT protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC2673 and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Anti-MAPT Monoclonal Antibody (V3S-1022-YC2673) in WB
Figure 3 Anti-MAPT Monoclonal Antibody (V3S-1022-YC2673) in ELISA
ELISA analysis of V3S-1022-YC2673 was performed by coating with human MAPT protein (His tag). Then blocked with BSA and incubated with V3S-1022-YC2673. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-MAPT Monoclonal Antibody (V3S-1022-YC2673) in ELISA
