Anti-NOTCH2 Neutralizing Antibody (V3S-0622-YC5128) (CAT#: V3S-0622-YC5128)

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    Figure 1 Anti-Human NOTCH2 Monoclonal Antibody (V3S-0622-YC5128) in FC






Description This product is a human monoclonal antibody that reacts with NOTCH2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application IF; IP; Neut; FuncS; ELISA; FC; WB
Application Notes The antibody is recommended for detection of NOTCH2 by IF, IP, Neut, FuncS, ELISA, FC, WB assays.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target NOTCH2
Alternative Name hN2; AGS2; HJCYS
Gene ID 4853
UniProt Q04721
Related pathway Notch signaling pathway
Research Area Neuroscience; Epigenetics

Tested Data


Figure 1 Anti-Human NOTCH2 Monoclonal Antibody (V3S-0622-YC5128) in FC

Flow cytometry analysis of U87 MG cells with purified antibody V3S-0622-YC5128 (QCAb249, Blue). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. Cells were then incubated with V3S-0622-YC5128 (5 μl/5x10^5 cells) for 60 min at 4°C. The secondary antibody used was FITC anti-human IgG antibody (3 μl) for 60 min at 4°C.

Figure 1 Anti-Human NOTCH2 Monoclonal Antibody (V3S-0622-YC5128) in FC

For research use only, not directly for clinical use.
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