| Description | This product is a human monoclonal antibody that reacts with NOTCH2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | IF; IP; Neut; FuncS; ELISA; FC; WB |
| Application Notes | The antibody is recommended for detection of NOTCH2 by IF, IP, Neut, FuncS, ELISA, FC, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human NOTCH2 Monoclonal Antibody (V3S-0622-YC5128) in FC
Flow cytometry analysis of U87 MG cells with purified antibody V3S-0622-YC5128 (QCAb249, Blue). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. Cells were then incubated with V3S-0622-YC5128 (5 μl/5x10^5 cells) for 60 min at 4°C. The secondary antibody used was FITC anti-human IgG antibody (3 μl) for 60 min at 4°C.
Figure 1 Anti-Human NOTCH2 Monoclonal Antibody (V3S-0622-YC5128) in FC
