Anti-NT5E Neutralizing Antibody (V3S-1022-YC377) (CAT#: V3S-1022-YC377)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant mouse antibody provided by CreativeBiolabs. The antibody is specific for 5'-Nucleotidase Ecto, the protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. It can be used for NT5E detection in Neutralization Assay, Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application Neut; FC

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target NT5E
Alternative Name 5'-Nucleotidase Ecto; 5-Nucleotidase Ecto; Ecto-5-Nucleotidase; EC 3.1.3.5; 5-NT; NT5; NTE; 5-Nucleotidase; Ecto (CD73); Purine 5-Prime-Nucleotidase; 5 Nucleotidase (CD73)
Gene ID 4907
UniProt P21589
Research Area Cardiovascular
Related Disease Cancer
For research use only, not directly for clinical use.
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