| Description | This product is a human antibody that recognizes NT5E. It blocks the degradation of AMP to immunosuppressive Ado, and effectively blocks the hydrolysis of immunogenic ATP into immunosuppressive adenosine. The antibody promotes antitumor immunity by stimulating dendritic cells and macrophages and restoring activation of T cells isolated from cancer patients. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Neutralization Mechanism | The mAb blocks the degradation of AMP to immunosuppressive Ado and blocks the hydrolysis of immunogenic ATP into immunosuppressive adenosine. |
| EC50 | 0.05 µg/mL (A375) 0.11 µg/mL (MDA-MB-231) |
| Affinity | KD = 0.14 nM |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Block; SPR |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
