| Description | This product is a Human monoclonal antibody that is capable of recognizing PDCD1. It can be used for PDCD1 detection in Western Blot (WB), Functional Assay (FuncS), Immunofluorescence (IF), Neutralization Assay (Neut), Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry (FC), Immunoprecipitation (IP). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | DB; WB; FuncS; IF; Neut; ELISA; FC; IP |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 ELISA analysis of anti-PD-1 antibody (V3S-1022-YC6030), Lot#CB2301J4.
ELISA analysis of anti-PD-1 antibody (Cat# V3S-1022-YC6030) was performed by coating with recombinant human PD-1 protein (His tag). Then blocked with BSA and incubated with anti-PD-1 antibodies. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 ELISA analysis of anti-PD-1 antibody (V3S-1022-YC6030), Lot#CB2301J4.
Figure 2 Western blot analysis of anti-PD-1 antibody (V3S-1022-YC6030), Lot#CB2301J4.
Western blot analysis of anti-PD-1 antibody (Cat# V3S-1022-YC6030) was performed by loading human PD-1 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with anti-PD-1 antibodies and HRP goat anti-human IgG was used as a secondary antibody (1/2000).
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Western blot analysis of anti-PD-1 antibody (V3S-1022-YC6030), Lot#CB2301J4.
Figure 3 Dot blot analysis of anti-PD-1 antibody (V3S-1022-YC6030), Lot#CB2301J4.
Dot blot analysis of anti-PD-1 antibody (Cat# V3S-1022-YC6030) was performed by coating with human PD-1 protein (His tag).
The anti-PD-1 antibody incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG was used as a secondary antibody (1/2000).
Figure 3 Dot blot analysis of anti-PD-1 antibody (V3S-1022-YC6030), Lot#CB2301J4.
