| Description | This product is a human monoclonal antibody that reacts with PDCD1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; IF; IP; Neut; FuncS; ELISA; FC; ICC |
| Application Notes | The antibody is recommended for detection of PDCD1 by IF, IP, Neut, FuncS, ELISA, FC, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 ELISA analysis of anti-PD-1 antibody (Cat# V3S-0622-YC5183).
ELISA analysis of anti-PD-1 antibody (Cat# V3S-0622-YC5183) was performed by coating with recombinant human PD-1 protein (His tag). Then blocked with BSA and incubated with anti-PD-1 antibodies. The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 ELISA analysis of anti-PD-1 antibody (Cat# V3S-0622-YC5183).
Figure 2 Western blot analysis of anti-PD-1 antibody (Cat# V3S-0622-YC5183).
Western blot analysis of anti-PD-1 antibody (Cat# V3S-0622-YC5183) was performed by loading human PD-1 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with anti-PD-1 antibodies and HRP goat anti-human IgG was used as a secondary antibody (1/2000).
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Western blot analysis of anti-PD-1 antibody (Cat# V3S-0622-YC5183).
Figure 3 Dot blot analysis of anti-PD-1 antibody (Cat# V3S-0622-YC5183).
Dot blot analysis of anti-PD-1 antibody (Cat# V3S-0622-YC5183) was performed by coating with human PD-1 protein (His tag).
The anti-PD-1 antibody incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG was used as a secondary antibody (1/2000).
Figure 3 Dot blot analysis of anti-PD-1 antibody (Cat# V3S-0622-YC5183).
