| Description | This product is a human neutralizing antibody clone iGL-CIS43.D3, which targets a junctional epitope of the Plasmodium falciparum circumsporozoite protein (PfCSP). The antibody iGL-CIS43.D3 has high affinity against PfCSP and it is significantly more potent than the current best-in-class PfCSP-directed antibody. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Plasmodium falciparum |
| Immunogen | Full length recombinant Plasmodium falciparum circumsporozoite protein |
| Epitope | It targets the peptide (KQPADGNPDPNANPNVDPN), a junctional epitope of the Plasmodium falciparum circumsporozoite protein. |
| Affinity | 1.01 nM, BLI-measured affinity for NPDP19 (KQPADGNPDPNANPNVDPN). 1.2 nM, ITC-measured affinity for a mutant form of PfCSP (PfCSPm). |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; BLI |
| Application Notes | ELISA: Wells were washed and incubated with 1:3 or 1:5 serially diluted antibody. Bio-layer interferometry (BLI): Association with antibody (serially diluted from 1000 to 62.5 nM) was done for 60s, followed by a dissociation step in buffer for 120s. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PfCSP |
| Alternative Name | Plasmodium falciparum circumsporozoite protein |
| Research Area | Infectious Disease |
| Related Disease | Malaria |
