Anti-RSPO1 (aa 31-263) Neutralizing Antibody (V3S-0622-YC5256) (CAT#: V3S-0622-YC5256)

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Description This product is a monoclonal antibody derived from mouse, which can specifically recognize RSPO1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Recombinant human RSPO1 protein amino acids 31-263
Epitope within the residues 31 to 263
Isotype IgG


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application WB; ELISA; Block; FC; IP
Application Notes The antibody is recommended for detection of RSPO1 by WB, ELISA, Block, FC, IP assays.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target RSPO1
Alternative Name RSPO1; R-spondin 1; RSPO; CRISTIN3; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1; anti-RSPO1
Gene ID 284654
UniProt Q2MKA7
Related pathway Wnt signaling pathway
Research Area Stem Cell; Developmental Biology
For research use only, not directly for clinical use.
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