| Description | The antibody C1A-F10 is a human mAb specific for SARS-CoV-2 Spike S1 protein. The IC50 value of antibody against SARS-CoV-2 is 0.184 μg/mL. The KD of antibody for RBD is 55.7 nM. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Affinity | 55.7 nM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | The IC50 value of C1A-F10 is 0.184 μg/mL. 1. Coat NUNC Maxisorp plates with His6-tagged SARS-CoV-2 S2P, SARS-CoV-2 RBD, or LUJV GP1 in PBS overnight at 4°C, followed by a blocking step with PBS containing 3% (v/v) BSA 0.02% (v/v) Tween. 2. Incubate antibody C1A-F10 at a concentration of 100 µg/ml for one hour. 3. Detect bound antibody with horseradish peroxidase (HRP)-coupled anti-human (Fc) antibody. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
