| Description | The antibody 910-30 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It has moderate neutralization capacity. It showed an IC50 of 0.071 μg/mL against a vesicular stomatitis virus SARS-CoV-2 pseudovirus and 0.142 μg/mL against authentic SARS-CoV-2. It recognized a glycan-independent epitope. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Recognized a glycan-independent epitope |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | S trimer and RBD ELISAs were performed in triplicate. 175 ng of antigen per well was coated onto 96-well ELISA plates at 4°C overnight. Plates were washed and then blocked with 100 μL of blocking buffer at 37°C for 2 hr. Purified antibodies were serial diluted using dilution buffer, added to the antigen-coated blocked plates, and then incubated at 4°C for 1 hr. Plates were washed and 50 μL of a secondary anti-human kappa light chain detection antibody was added to each well and incubated at room temperature for 1 hr. After the final wash, 50 μL TMB substrate was used to detect antibody binding to antigen measuring absorbance at 405 nm. The antibody 910-30 showed an IC50 of 0.071 μg/mL against a vesicular stomatitis virus SARS-CoV-2 pseudovirus and 0.142 μg/mL against authentic SARS-CoV-2. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
