| Description | The antibody P17 is a human mAb specific for SARS-CoV-2 Spike S1 protein. The antibody showed tight binding to SARS-CoV-2 RBD with a EC50 of 29 pM by the enzyme-linked immunosorbent assay. But it did not interact with SARS-CoV RBD, suggesting that antibody is SARS-CoV-2 specific. The antibody prevented SARS-CoV-2 infection in a dose dependent manner in Vero cells. It exhibited stronger neutralizing activity with IC50 and PRNT50 values of 165 and 195 pM, respectively. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | SARS-CoV-2 Spike RBD-his protein or mutants was coated in 96-well plate at 4°C overnight. The 96-well plate was then blocked with PBS containing 5% non-fat milk powder for 1 h. Serial dilutions of P17 antibody were added into the plate and incubated for 2 h at room temperature. After washing away the excess antibodies, the HRP-labeled goat anti human Fc secondary antibody was added and incubated for another 1 h. The plate was washed by PBST, and then incubated with HRP substrate TMB for color development and quenched by adding 40 μL 2 M HCl. Finally, the binding ability was revealed by the absorbance at 450 nM wavelength using the plate reader. P17 exhibited stronger neutralizing activity with IC50 and PRNT50 values of 165 and 195 pM, respectively. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
