| Description | The antibody D2 is a human mAb specific for SARS-CoV-2 Spike S1 protein. The antibody exhibited high-affinity binding to Delta-RBD, Omicron-RBD-BA.1 and Omicron RBD-BA.2. It retained high-affinity binding at pM level to Delta-RBD (KD = ~62 pM), Omicron-RBD-BA.1 (KD = ~5.45 pM) and Omicron-RBD-BA.2 (KD = ~5.22 pM), and the binding to Delta-RBD is slightly weaker than that to Omicron-RBD. It was able to potently neutralize SARS-CoV-2 WT pseudovirus with IC50 of 19 ng/mL, and it retained the same-level high potencies in neutralizing pseudoviruses of Alpha, Beta, Delta and Omicron including BA.1, BA.2 and BA.3 with IC50 values less than 50 ng/mL. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | Delta, Omicron BA.1 and BA.2 |
| Epitope | Receptor-binding domain (RBD) |
| Affinity | Delta-RBD (KD = 62 pM), Omicron-RBD-BA.1 (KD = 5.45 pM) and Omicron-RBD-BA.2 (KD = 5.22 pM). |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | The antibody was able to potently neutralize SARS-CoV-2 WT pseudovirus with IC50 of 19 ng/mL. ELISA plates were coated with SARS-CoV-2 protein including WT-S1, WT-NTD, WT-RBD, Delta-S1, Delta_x0002_RBD and Omicron-RBD at 4°C overnight. Following washing with PBST, serial dilutions of testing antibodies starting at 5 μg/mL were added to each well and incubated at 37°C for 30 min. After washing with PBST, HRP-conjugated anti-human IgG Fc specific antibody was added at the dilution of 1:2000 and incubated at 37°C for 30 min. The absorbance was detected at 450 nm. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
