| Description | The anti-SARS-CoV-2 antibody is a mouse IgG, clone WS6. This product is a recombinant antibody provided by Creative Biolabs. It recognizes diverse beta-coronaviruses spikes and neutralizes SARS-CoV-2 and variants, SARS-CoV, and related viruses from bat, pangolin, and other animals. It neutralizes by inhibition of fusion steps post-viral attachment. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | SARS-CoV variants, related viruses from bat and pangolin |
| Epitope | S2 subunit |
| Function | Neutralizes SARS-CoV-2, SARS-CoV variants and related viruses from bat, pangolin. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | ELISA plates were coated with the SARS-CoV-2 proteins, S-dTM, S1-short, S1R, RBD, and NTD, at 1 μg/ml in PBS at 4°C overnight. After standard washes and blocks, plates were incubated with 50 ml serial dilutions of antibody in each well for one hour at room temperature. Anti-mouse whole IgG horseradish peroxidase conjugates were used as secondary antibodies, and TMB was used as the substrate. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
