Recombinant Anti-SEMA4D Antibody (V3S-1022-YC1196) (CAT#: V3S-1022-YC1196)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant human antibody provided by CreativeBiolabs. The antibody is specific for Semaphorin 4D, the protein is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. It can be used for SEMA4D detection in Enzyme-Linked Immunosorbent Assay, Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Human
Affinity The affinity is in nanomolar range, about 0.13 nM, determined by surface plasmon resonance.
Isotype IgG4

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA; FC

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target SEMA4D
Alternative Name Semaphorin 4D; Sema Domain; Immunoglobulin Domain (Ig); Transmembrane Domain (TM) And Short Cytoplasmic Domain; (Semaphorin) 4D; C9orf164; SEMAJ; CD100; BB18; GR3; A8; Sema Domain; Immunoglobulin Domain (Ig); Transmembrane Domain (TM) And Short Cytoplasmic Domain; 4D
Gene ID 10507
UniProt Q92854
Research Area Signal Pathway
For research use only, not directly for clinical use.
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