Anti-SEMA4D (ECD domain) Neutralizing Antibody (V3S-0522-YC2734) (CAT#: V3S-0522-YC2734)

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Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Semaphorin 4D. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human, Mouse
Immunogen Purified mouse CD100-His (the extracellular domain of mouse CD100 with a C-terminal 6× his tag
Epitope Within the ECD domain.
Affinity KD = 1.2 nM (Human SEMA4D), 1.0 nM (Mouse SEMA4D), determined by surface plasmon resonance.
Isotype IgG2b


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application ELISA; Block; FuncS
Application Notes The antibody is recommended for detection of SEMA4D by ELISA, Block, FuncS assays.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target SEMA4D
Alternative Name Semaphorin 4D; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, (Semaphorin) 4D; C9orf164; SEMAJ; CD100; BB18; GR3; A8; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, 4D
Gene ID 10507
UniProt Q92854
Research Area Immunology; Neuroscience
For research use only, not directly for clinical use.
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