| Description | This product is a recombinant human antibody provided by CreativeBiolabs. The antibody is specific for Synaptosome Associated Protein 25, Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). It can be used for SNAP25 detection in Western Blot, Immunohistochemistry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; WB; IHC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SNAP25 |
| Alternative Name | Synaptosome Associated Protein 25; Resistance To Inhibitors Of Cholinesterase 4 Homolog; Synaptosomal-Associated Protein; 25kDa; SNAP-25; SNAP; SUP; Synaptosomal-Associated 25 KDa Protein; Synaptosome Associated Protein 25kDa; Synaptosomal-Associated Protein 25 |
| Gene ID | 6616 |
| UniProt | P60880 |
| Research Area | Neuroscience |
Figure 1 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SDS-PAGE
SDS-PAGE analysis of V3S-1022-YC419 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SDS-PAGE
Figure 2 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SEC-HPLC
The purity of V3S-1022-YC419 was greater than 98% as determined by HPLC.
Figure 2 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SEC-HPLC
Figure 3 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in ELISA
ELISA analysis of V3S-1022-YC419 was performed by coating with Human SNAP25 Protein (His Tag).
The secondary antibody: HRP-goat-Anti-Human IgG (H+L)
Figure 3 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in ELISA
Figure 4 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in DB
Dot Blot analysis of V3S-1022-YC419 was performed by coating with Human SNAP25 Protein (His Tag).
V3S-1022-YC419 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Figure 4 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in DB
