Recombinant Anti-SNAP25 Antibody (V3S-1022-YC419)

Figure 1 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SDS-PAGE
Figure 2 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SEC-HPLC
Figure 3 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in ELISA
Figure 4 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in DB

Datasheet

MSDS

COA

Summary

Description	This product is a recombinant human antibody provided by CreativeBiolabs. The antibody is specific for Synaptosome Associated Protein 25, Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). It can be used for SNAP25 detection in Western Blot, Immunohistochemistry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality	Monoclonal
Host Species	Human
Target Species	Human
Isotype	IgG1

Property

Expression Species	HEK293F or CHO Cell line
Conjugation	Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity	>95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin	<1 EU/mg, determined by LAL method
Purification	Purified with Protein A or G affinity chromatography
Sterility	0.2 μM filtered
Formulation	PBS, pH 7.4
Preservation	No preservatives
Stabilizer	No stabilizers
Storage	Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Tested Data

SDS-PAGE

Figure 1 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SDS-PAGE

SDS-PAGE analysis of V3S-1022-YC419 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 1 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SDS-PAGE

SEC-HPLC

Figure 2 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SEC-HPLC

The purity of V3S-1022-YC419 was greater than 98% as determined by HPLC.

Figure 2 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in SEC-HPLC

ELISA

Figure 3 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in ELISA

ELISA analysis of V3S-1022-YC419 was performed by coating with Human SNAP25 Protein (His Tag).

The secondary antibody: HRP-goat-Anti-Human IgG (H+L)

Figure 3 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in ELISA

Figure 4 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in DB

Dot Blot analysis of V3S-1022-YC419 was performed by coating with Human SNAP25 Protein (His Tag).

V3S-1022-YC419 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)

Figure 4 Anti-SNAP25 Monoclonal Antibody (V3S-1022-YC419) in DB

ELISA	Enzyme-Linked Immunosorbent Assay Protocol
WB	Western Blot Protocol
FC	Flow Cytometry Protocol

Target	SNAP25
Alternative Name	Synaptosome Associated Protein 25; Resistance To Inhibitors Of Cholinesterase 4 Homolog; Synaptosomal-Associated Protein; 25kDa; SNAP-25; SNAP; SUP; Synaptosomal-Associated 25 KDa Protein; Synaptosome Associated Protein 25kDa; Synaptosomal-Associated Protein 25
Gene ID	6616
UniProt	P60880
Research Area	Neuroscience

Recombinant Anti-SNAP25 Antibody (V3S-1022-YC419) (CAT#: V3S-1022-YC419)

Summary

Property

Applications

Protocols

Target

Tested Data

Recombinant Anti-SNAP25 Antibody (V3S-1022-YC419) (CAT#: V3S-1022-YC419)

Summary

Property

Applications

Protocols

Target

Tested Data

Isotype Control

Secondary Antibody

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