| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Synaptosome Associated Protein 25. It can be used for Synaptosome Associated Protein 25 detection in Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunoprecipitation and Immunofluorescence. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Rat |
| Epitope | The epitope is in the EANQ. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; ICC; IP; IF |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-SNAP25 Antibody (V3S-1022-YC2297) in ELISA.
ELISA analysis of V3S-1022-YC2297 was performed by coating with recombinant human SNAP-25 protein (his Tag). Then blocked with BSA and incubated with anti-human SNAP25 Antibody (V3S-1022-YC2297). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-SNAP25 Antibody (V3S-1022-YC2297) in ELISA.
Figure 2 Anti-SNAP25 Antibody (V3S-1022-YC2297) in Western Blot.
Western blot analysis of V3S-1022-YC2297 was performed by loading recombinant human SNAP-25 protein (his Tag) onto a 12% Tris-HCl polyacrylamide gel. Membranes were probed with V3S-1022-YC2297 and HRP goat anti-human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Anti-SNAP25 Antibody (V3S-1022-YC2297) in Western Blot.
Figure 3 Anti-SNAP25 Antibody (V3S-1022-YC2297) in Dot Blot.
Dot blot analysis of V3S-1022-YC2297 was performed by coating with recombinant human SNAP-25 protein (his Tag).
V3S-1022-YC2297 incubation concentration: 1 ng/μL.
The secondary antibody: HRP-anti-human IgG (H+L)
Figure 3 Anti-SNAP25 Antibody (V3S-1022-YC2297) in Dot Blot.
