| Description | This product is a human monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing human alpha-synuclein (SNCA). It can be used for SNCA detection in Immunoprecipitation, Western Blot and Functional Assay. The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Epitope | AA 118-126 of α-synuclein |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A or G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | IP; WB; FuncS |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SNCA |
| Alternative Name | Synuclein, Alpha (Non A4 Component Of Amyloid Precursor); PARK1; NACP; Parkinson Disease (Autosomal Dominant, Lewy Body); Non-A4 Component Of Amyloid Precursor; Non A-Beta Component Of AD Amyloid; Non-A Beta Component Of AD Amyloid; Synuclein Alpha-140 |
| Gene ID | 6622 |
| UniProt | P37840 |
| Research Area | Cancer Research |
Figure 1 Human Anti-SNCA Monoclonal Antibody (V3S-0822-YC3127) in WB
Western blot analysis of V3S-0822-YC3127 was performed by loading non-reducing (lane 1, 0.06 µg) and reducing (lane 2, 0.06 µg) form of SNCA antigen onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0822-YC3127 (2 µg/mL) and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 1 Human Anti-SNCA Monoclonal Antibody (V3S-0822-YC3127) in WB
Figure 2 Human Anti-SNCA Monoclonal Antibody (V3S-0822-YC3127) in ELISA
ELISA analysis of V3S-0822-YC3127 was performed by coating with SNCA antigen (1 µg/mL). Then blocked with BSA, and incubated with V3S-0822-YC3127. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Human Anti-SNCA Monoclonal Antibody (V3S-0822-YC3127) in ELISA
