| Description | This product is a monoclonal antibody derived from rabbit, which can specifically recognize SNCA. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Target Species | Human |
| Immunogen | Mixture of alpha-synuclein C 1 oligomers, alpha-synuclein fibrils and alpha-synuclein TP mutant oligomers |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FuncS |
| Application Notes | The antibody is recommended for detection of SNCA by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Rabbit Anti-SNCA Monoclonal Antibody (V3S-0622-YC2572) in WB
Western blot analysis of V3S-0622-YC2572 was performed by loading human recombinant alpha-synuclein protein.
V3S-0622-YC2572 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Rabbit IgG (H+L)
Lane 1: Reducing Antigen (1 μg)
Figure 1 Rabbit Anti-SNCA Monoclonal Antibody (V3S-0622-YC2572) in WB
Figure 2 Rabbit Anti-SNCA Monoclonal Antibody (V3S-0622-YC2572) in DB
Dot blot analysis of V3S-0622-YC2572 was performed by loading human recombinant alpha-synuclein protein.
V3S-0622-YC2572 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Rabbit IgG (H+L)
Figure 2 Rabbit Anti-SNCA Monoclonal Antibody (V3S-0622-YC2572) in DB
Figure 3 Rabbit Anti-SNCA Monoclonal Antibody (V3S-0622-YC2572) in ELISA
ELISA analysis of V3S-0622-YC2572 was performed by coating with human recombinant alpha-synuclein protein.
The secondary antibody: HRP-Anti-Rabbit IgG (H+L)
Figure 3 Rabbit Anti-SNCA Monoclonal Antibody (V3S-0622-YC2572) in ELISA
