| Description | This chicken anti-ZIKV E IgY antibody (clone ZIKVEF2L01) recognizes recombinant ZIKV E proteins. White leghorn chicken were immunized with a truncated recombinant E protein of Zika virus. The ZIKVEF2L01 scFv showed antigen recognition against both truncated proteins fragment 2 (F2, aa 1-251) and fragment 3 (F3, aa 220-366). It had lower binding activity against F3 protein but moderate reactivity against F2 protein. |
| Clonality | Monoclonal |
| Host Species | Chicken |
| Target Species | Zika virus (ZIKV) |
| Immunogen | ZIKV E recombinant proteins with His-tag |
| Epitope | Fragment 2 (aa 1-251) and fragment 3 (aa 220-366) from ZIKV E protein (aa 1-505). |
| Affinity | KD = 0.2 nM |
| Function | Envelope protein E: Binds to host cell surface receptors and mediates fusion between viral and cellular membranes. Efficient virus attachment to cell is, at least in part, mediated by host HAVCR1 in a cell-type specific manner. In addition, host NCAM1 can also be used as entry receptor. Interaction with host HSPA5 plays an important role in the early stages of infection as well. Envelope protein is synthesized in the endoplasmic reticulum and forms a heterodimer with protein prM. The heterodimer plays a role in virion budding in the ER, and the newly formed immature particle is covered with 60 spikes composed of heterodimers between precursor prM and envelope protein E. The virion is transported to the Golgi apparatus where the low pH causes the dissociation of PrM-E heterodimers and formation of E homodimers. PrM-E cleavage is inefficient, many virions are only partially matured and immature prM-E proteins could play a role in immune evasion. |
| Isotype | Chicken IgY |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; FC |
| Application Notes | The results of ELISA showed the scFv had lower binding activity against ZIKV E F3 protein but moderate reactivity against ZIKV E F2 protein. Western blot detected with mouse anti-HA tag antibody, followed by rabbit anti-mouse IgG HRP-conjugated antibody to confirm purified antibody. Flow cytometry showed that the scFv recognized ZIKV E protein in a transfected Vero cell. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ZIKV E |
| Alternative Name | ZIKV_gp1 |
| Gene ID | 7751225 |
| Research Area | Microbiology |
| Related Disease | Meningoencephalitis; Myelitis; Guillain-Barré syndrome; Microcephaly |
| Post Translational Modification | Envelope protein E: N-glycosylation plays a role in virulence in mammalian and mosquito hosts, but may have no effect on neurovirulence. Ubiquitination by host TRIM7 promotes virus attachment and fusion of the virus and the host endosome membrane. |
