| Sub Cat | Host Species | Immunogen | Isotype | Application | |
|---|---|---|---|---|---|
| V3S-1124-YC119 | Rat | IgG | ELISA; FC; IP; FuncS; IF; Neut; ICC | ||
| V3S-0622-YC81 | Rat | Human CD4+ T cells | IgG | FC; WB; Inhib | |
| V3S-0522-YC44 | Mouse | IgG1 | ELISA; IF; IP | ||
| V3S-0822-YC2681 | Hamster | Mouse BM10-37 cytotoxic T cells | IgG1 | Dep; Stim; Activ; IF; FC; WB | |
| V3S-0822-YC2682 | Rat | CBAT6 mouse thymocytes | IgG2a | Stim; Activ; IF | |
| V3S-0822-YC53 | Hamster | IgG2a kappa | FC | ||
| V3S-0822-YC55 | Hamster | Mouse T-cell receptor | IgG2a kappa | IHC-Fr; FC; IP; FuncS |
| Description | This product is a monoclonal antibody derived from rat, which can specifically recognize CD3E. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Rat |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; IP; FuncS; IF; Neut; ICC |
| Application Notes | The antibody is recommended for detection of CD3E by ELISA, FC, IP, FuncS, IF, Neut, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD3E |
| Alternative Name | CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T- |
| Gene ID | 916 |
| UniProt | P07766 |
| Related pathway | G-protein signaling N-RAS regulation pathway; Toll-like receptor signaling pathway |
| Research Area | Immunology; Stem Cell |
