| Description | This product is a monoclonal antibody specifically binding to human CD3 epsilon. The antibody has cross reaction with human and cynomolgus CD3E. It can bind to human and cynomolgus T cells, activates human T cells and does not bind to the same epitope as the antibody clone OKT3, UCHT1 and SP34. |
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Target Species | Human, Cynomolgus |
| Immunogen | Recombinant human and cynomolgus CD3g(G4S)5CD3e-AcTev-Fc(knob)-Avi protein |
| Epitope | The epitope is located in the amino acids of 77-96 (human) and 69-88 (monkey). |
| Affinity | Human CD3E (6.1 μM), cynomolgus CD3E (0.62 μM) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FC; FuncS |
| Application Notes | Binding of anti-CD3 antibody clone 645 to N- and C-terminally biotinylated human and cynomolgus CD3 peptides was determined by ELISA. Results show that clone 645 does not bind to human CD3 peptide 77-96 that is immobilized on a streptavidin-coated microplate via C-terminally fused biotin. However, clone 645 binds the same peptide when immobilized N-terminally as well as both cynomolgus CD3 peptides 69-88 irrespective of the site of biotin fusion. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD3E |
| Alternative Name | T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain |
| Gene ID | 916 |
| UniProt | P07766 |
| Related pathway | G-protein signaling N-RAS regulation pathway; Toll-like receptor signaling pathway |
| Research Area | Immunology; Stem Cell |
